4-bromooxane

反応条件: temp:0.00; pressure:0.00; stir:

反応条件: temp:0.00; pressure:0.00; stir:

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A solution of 4-bromo-tetrahydro-pyran (0.82g, 5 mmol) in dry THF (10 mL) was added dropwise to the suspension of magnesium (132 mg, 5.5 mmol) and iodine (25 mg) in dry THF (20 mL) at 50° C. under N2. The mixture was stirred for 30 min after addition at 50° C., then cooled to room temperature. Then a THF (10 mL) solution of 3-(5-amino-4-cyano-imidazol-1-yl)-N-cyclopropyl-4-methyl-benzamide (90 mg, 0.32 mmol) was added to the reaction mixture and it was stirred at room temperature for 3h then quenched with HCl (2N) and stirred at room temperature overnight. The pH of the solution was adjusted pH 8 with saturated aqueous K2CO3 was and it was extracted with EtOAc. The organic layer was washed by water and brine, dried over Na2SO4, and concentrated. The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1), and the product was obtained as a beige solid (35 mg, 30%). HPLC (4 minute gradient) tR=3.05 min; MS m/z 369.18 [M+H]+. after addition at 50° C. then quenched with HCl (2N) stirred at room temperature overnight it was extracted with EtOAc The organic layer was washed by water and brine dried over Na2SO4 concentrated The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1)

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A solution of 4-bromo-tetrahydro-pyran (0.82g, 5 mmol) in dry THF (10 mL) was added dropwise to the suspension of magnesium (132 mg, 5.5 mmol) and iodine (25 mg) in dry THF (20 mL) at 50° C. under N2. The mixture was stirred for 30 min after addition at 50° C., then cooled to room temperature. Then a THF (10 mL) solution of 3-(5-amino-4-cyano-imidazol-1-yl)-N-cyclopropyl-4-methyl-benzamide (90 mg, 0.32 mmol) was added to the reaction mixture and it was stirred at room temperature for 3h then quenched with HCl (2N) and stirred at room temperature overnight. The pH of the solution was adjusted pH 8 with saturated aqueous K2CO3 was and it was extracted with EtOAc. The organic layer was washed by water and brine, dried over Na2SO4, and concentrated. The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1), and the product was obtained as a beige solid (35 mg, 30%). HPLC (4 minute gradient) tR=3.05 min; MS m/z 369.18 [M+H]+. after addition at 50° C. then quenched with HCl (2N) stirred at room temperature overnight it was extracted with EtOAc The organic layer was washed by water and brine dried over Na2SO4 concentrated The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1)

反応条件: temp:80.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: To a 100 mL round-bottomed flask containing zinc dust (2.03 g, 31.0 mmol, 1.70 equiv) in N,N-dimethylacetamide (20 mL) was added chlorotrimethylsilane (0.473 mL, 3.65 mmol, 0.200 equiv) and 1,2-dibromoethane (0.318 mL, 3.65 mmol, 0.200 equiv) over 10 min. A solution of 4-bromotetrahydro-2H-pyran (2.88 mL, 25.6 mmol, 1.40 equiv) in N,N-dimethylacetamide (15 mL) was then added to the mixture. After 30 min, the reaction mixture was filtered over Celite and added to a round-bottomed flask containing [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (745 mg, 0.913 mmol, 0.0500 equiv), cuprous iodide (348 mg, 1.83 mmol, 0.100 equiv), and 2,6-dichloro-4-iodo-pyridine (5.00 g, 18.3 mmol, 1.00 equiv). The reaction was then heated to 80° C. for 16 h. After cooling to room temperature, the reaction mixture was filtered over Celite washing with ethyl acetate (3×10 mL) and concentrated to dryness. Purification by flash column chromatography (30:70 ethyl acetate/heptane) furnished the product as a white solid (3.00 g, 71% yield). 1H NMR (CDCl3, 400 MHz), δ: 7.11 (s, 1H), 4.09 (dt, J=11.6, 3.3 Hz, 2H), 3.57-3.42 (m, 2H), 2.85-2.70 (m, 1H), 1.77 (tt, J=7.5, 3.7 Hz, 4H). the reaction mixture was filtered over Celite added to a round-bottomed flask After cooling to room temperature the reaction mixture was filtered over Celite washing with ethyl acetate (3×10 mL) concentrated to dryness Purification by flash column chromatography (30:70 ethyl acetate/heptane)

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A solution of 4-bromo-tetrahydro-pyran (0.82 g, 5 mmol) in dry THF (10 mL) was added dropwise to the suspension of magnesium (132 mg, 5.5 mmol) and iodine (25 mg) in dry THF (20 mL) at 50° C. under N2. The mixture was stirred for 30 min after addition at 50° C., then cooled to room temperature. Then a THF (10 mL) solution of 3-(5-amino-4-cyano-imidazol-1-yl)-N-cyclopropyl-4-methyl-benzamide (90 mg, 0.32 mmol) was added to the reaction mixture and it was stirred at room temperature for 3 h then quenched with HCl (2N) and stirred at room temperature overnight. The pH of the solution was adjusted pH ˜8 with saturated aqueous K2CO3 was and it was extracted with EtOAc. The organic layer was washed by water and brine, dried over Na2SO4, and concentrated. The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1), and the product was obtained as a beige solid (35 mg, 30%). HPLC (4 minute gradient) tR=3.05 min; MS m/z 369.18 [M+H]+. after addition at 50° C. was stirred at room temperature for 3 h then quenched with HCl (2N) stirred at room temperature overnight it was extracted with EtOAc The organic layer was washed by water and brine dried over Na2SO4 concentrated The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1)

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A solution of 4-bromo-tetrahydro-pyran (0.82 g, 5 mmol) in dry THF (10 mL) was added dropwise to the suspension of magnesium (132 mg, 5.5 mmol) and iodine (25 mg) in dry THF (20 mL) at 50° C. under N2. The mixture was stirred for 30 min after addition at 50° C., then cooled to room temperature. Then a THF (10 mL) solution of 3-(5-amino-4-cyano-imidazol-1-yl)-N-cyclopropyl-4-methyl-benzamide (90 mg, 0.32 mmol) was added to the reaction mixture and it was stirred at room temperature for 3 h then quenched with HCl (2N) and stirred at room temperature overnight. The pH of the solution was adjusted pH ˜8 with saturated aqueous K2CO3 was and it was extracted with EtOAc. The organic layer was washed by water and brine, dried over Na2SO4, and concentrated. The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1), and the product was obtained as a beige solid (35 mg, 30%). HPLC (4 minute gradient) tR=3.05 min; MS m/z 369.18 [M+H]+. after addition at 50° C. was stirred at room temperature for 3 h then quenched with HCl (2N) stirred at room temperature overnight it was extracted with EtOAc The organic layer was washed by water and brine dried over Na2SO4 concentrated The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1)

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A solution of 4-bromo-tetrahydro-pyran (0.82g, 5 mmol) in dry THF (10 mL) was added dropwise to the suspension of magnesium (132 mg, 5.5 mmol) and iodine (25 mg) in dry THF (20 mL) at 50° C. under N2. The mixture was stirred for 30 min after addition at 50° C., then cooled to room temperature. Then a THF (10 mL) solution of 3-(5-amino-4-cyano-imidazol-1-yl)-N-cyclopropyl-4-methyl-benzamide (90 mg, 0.32 mmol) was added to the reaction mixture and it was stirred at room temperature for 3h then quenched with HCl (2N) and stirred at room temperature overnight. The pH of the solution was adjusted pH 8 with saturated aqueous K2CO3 was and it was extracted with EtOAc. The organic layer was washed by water and brine, dried over Na2SO4, and concentrated. The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1), and the product was obtained as a beige solid (35 mg, 30%). HPLC (4 minute gradient) tR=3.05 min; MS m/z 369.18 [M+H]+. after addition at 50° C. then quenched with HCl (2N) stirred at room temperature overnight it was extracted with EtOAc The organic layer was washed by water and brine dried over Na2SO4 concentrated The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1)

反応条件: temp:0.00; pressure:0.00; stir:

反応条件: temp:0.00; pressure:0.00; stir:

反応条件: temp:90.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: 4-Bromotetrahydropyran (Frontier Scientific #B10197, 25.8 g, 156 mmol) was added to the solution of sodium hydroxide (16.0 g, 400 mmol) in 40 mL water. The biphase mixture was stirred in 90° C. with a condenser for 1 day. The mixture was cooled to RT and transferred to a small separatory funnel. The organic phase was carefully separated. It was a mixture of alkene J79 (major) and leftover 4-bromotetrahydropyran. This mixture was dissolved in 100 mL chloroform. To it was added MCPBA (77%, 26.8 g, 120 mmol) in small portions. The mixture was vigorously stirred at RT for overnight. The thick suspension was chilled in ice bath and filtered through celite. The solid cake was washed with ice-chilled chloroform 500 mL×3. All the filtrate and wash solutions were transferred into a big reparatory funnel. It was washed with saturated NaHCO3 solution twice. The organic phase was then dried over anhydrous Na2SO3 (30 g). The mixture was filtered and the filtrate was concentrated on a rotary instrument in ice-bath bath (<5° C.) to remove chloroform. A light liquid (J80) with some remaining meta-chlorobenzoic acid solid was obtained. This mixture was dissolved in 100 mL isopropanol. To it was added (S)-(−)-α-methylbenzylamine (15.2 mL, 120 mmol). A gel-like mixture was got initially. The mixture was slowly heated to 70° C. and stirred. A clean solution was then got. The clear solution was stirred for 2 days at 70° C. It was then concentrated in vacuo. The residue was taken into 500 mL EtAOc and washed with saturated Na2CO3 solution three times. The organic phase was dried, concentrated and subjected to flash column (0-40% EtOAc in DCM) to isolate desired product J81 (3.52 g) as a cream-colored solid. UV: 259 nm. M+H found for C13H19NO2: 222.2. NMR (DMSO-d6): 7.32-7.25 (4H, m), 7.18 (1H, t), 4.89 (1H, d), 3.86 (1H, m), 3.63 (1H, m), 3.45-3.37 (2H, m, partially obscured by water), 3.19 (1H, m), 2.66 (1H, m), 2.22 (1H, m), 1.92 (1H, m), 1.74 (1H, d), 1.35 (1H, m), 1.22 (3H, d, J=6.4 Hz) ppm. The mixture was cooled to RT transferred to a small separatory funnel The organic phase was carefully separated a mixture of alkene J79 (major) and leftover 4-bromotetrahydropyran This mixture was dissolved in 100 mL chloroform The mixture was vigorously stirred at RT for overnight The thick suspension was chilled in ice bath filtered through celite The solid cake was washed with ice- chilled chloroform 500 mL×3 All the filtrate and wash solutions were transferred into a big reparatory funnel It was washed with saturated NaHCO3 solution twice The organic phase was then dried over anhydrous Na2SO3 (30 g) The mixture was filtered the filtrate was concentrated on a rotary instrument in ice-bath bath (<5° C.) to remove chloroform

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A solution of 4-bromo-tetrahydro-pyran (0.82 g, 5 mmol) in dry THF (10 mL) was added dropwise to the suspension of magnesium (132 mg, 5.5 mmol) and iodine (25 mg) in dry THF (20 mL) at 50° C. under N2. The mixture was stirred for 30 min after addition at 50° C., then cooled to room temperature. Then a THF (10 mL) solution of 3-(5-amino-4-cyano-imidazol-1-yl)-N-cyclopropyl-4-methyl-benzamide (90 mg, 0.32 mmol) was added to the reaction mixture and it was stirred at room temperature for 3 h then quenched with HCl (2N) and stirred at room temperature overnight. The pH of the solution was adjusted pH ˜8 with saturated aqueous K2CO3 was and it was extracted with EtOAc. The organic layer was washed by water and brine, dried over Na2SO4, and concentrated. The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1), and the product was obtained as a beige solid (35 mg, 30%). HPLC (4 minute gradient) tR=3.05 min; MS m/z 369.18 [M+H]+. after addition at 50° C. was stirred at room temperature for 3 h then quenched with HCl (2N) stirred at room temperature overnight it was extracted with EtOAc The organic layer was washed by water and brine dried over Na2SO4 concentrated The crude product was purified by column chromatography on silical gel (EtOAc˜EtOAc:MeOH:Et3N=100:10:1)