ethyl 5-oxo-1,4-dihydropyrazole-3-carboxylate

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: Tert-butyldimethylsilyl chloride (6.05 g, 40.15 mmol) and imidazole (2.92 g, 42.82 mmol) were added to a stirred solution of ethyl 5-oxo-4,5-dihydro-1H-pyrazole-3-carboxylate (4.18 g, 26.76 mmol) in ACN (90 mL). The mixture was stirred at room temperature for 30 minutes, then diluted with water and extracted with AcOEt. The organic layer was separated, dried (Na2SO4), filtered and the solvents evaporated in vacuo to yield ethyl 5-{[tert-butyl(dimethyl)silyl]oxy}-1H-pyrazole-3-carboxylate (3.2 g, 44% yield) as a yellow solid, that was used in the next step without further purification. extracted with AcOEt The organic layer was separated dried (Na2SO4) filtered the solvents evaporated in vacuo

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: Tert-butyldimethylsilyl chloride (6.05 g, 40.15 mmol) and imidazole (2.92 g, 42.82 mmol) were added to a stirred solution of ethyl 5-oxo-4,5-dihydro-1H-pyrazole-3-carboxylate (4.18 g, 26.76 mmol) in ACN (90 mL). The mixture was stirred at room temperature for 30 minutes, then diluted with water and extracted with AcOEt. The organic layer was separated, dried (Na2SO4), filtered and the solvents evaporated in vacuo to yield ethyl 5-{[tert-butyl(dimethyl)silyl]oxy}-1H-pyrazole-3-carboxylate (3.2 g, 44% yield) as a yellow solid, that was used in the next step without further purification. extracted with AcOEt The organic layer was separated dried (Na2SO4) filtered the solvents evaporated in vacuo

反応条件: temp:90.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A stirred suspension of 5.3 g of a 50% mineral oil dispersion of sodium hydride in 30 ml of dimethylformamide is treated dropwise at -10° C., under a nitrogen atmosphere, with a solution of 15.6 g of 5-oxo-2-pyrazoline-3-carboxylic acid, ethyl ester, in 65 ml of dimethylformamide. After the evolution of hydrogen ceases, the mixture is stirred for 2 hours while allowing the temperature to rise to +10° C. The mixture is recooled to -10° C. and treated dropwise, with stirring, with a solution of 21.6 g of 90% N-methylisatoic anhydride in 150 ml of dimethylformamide, then stirred for 18 hours while allowing the temperature to rise to 20°-25° C. The mixture is heated at 90° C. for 1 hour, cooled and poured into 3 liters of ice water containing 10 ml of concentrated hydrochloric acid (final pH about 5). The resulting precipitate is collected by filtration and the filtrate is extracted several times with 600 ml portions of dichloromethane. The precipitate is dissolved in 1 liter of dichloromethane and the solution is combined with the above dichloromethane extracts. The combined dichloromethane solution is washed with water, dried and evaporated. The residual oil is triturated with methanol and the resulting solid is collected, dissolved in tetrahydrofuran and the solution is decolorized by passage through an activated magnesium silicate column (Florisil R). The filtrate is concentrated to a small volume, diluted with ethanol and chilled. The resulting crystalline precipitate of 4,9-dihydro-4-methyl-9-oxo-pyrazolo[5,1-b]quinazoline-2-carboxylic acid, ethyl ester is collected by filtration; mp 239°-241° C., after recrystallization from dichloromethane/ethanol. to rise to +10° C is recooled to -10° C. treated dropwise to rise to 20°-25° C cooled The resulting precipitate is collected by filtration the filtrate is extracted several times with 600 ml portions of dichloromethane The precipitate is dissolved in 1 liter of dichloromethane The combined dichloromethane solution is washed with water dried evaporated The residual oil is triturated with methanol the resulting solid is collected dissolved in tetrahydrofuran The filtrate is concentrated to a small volume diluted with ethanol chilled The resulting crystalline precipitate of 4,9-dihydro-4-methyl-9-oxo-pyrazolo[5,1-b]quinazoline-2-carboxylic acid, ethyl ester is collected by filtration mp 239°-241° C., after recrystallization from dichloromethane/ethanol

反応条件: temp:90.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A stirred suspension of 5.3 g of a 50% mineral oil dispersion of sodium hydride in 30 ml of dimethylformamide is treated dropwise at -10° C., under a nitrogen atmosphere, with a solution of 15.6 g of 5-oxo-2-pyrazoline-3-carboxylic acid, ethyl ester, in 65 ml of dimethylformamide. After the evolution of hydrogen ceases, the mixture is stirred for 2 hours while allowing the temperature to rise to +10° C. The mixture is recooled to -10° C. and treated dropwise, with stirring, with a solution of 21.6 g of 90% N-methylisatoic anhydride in 150 ml of dimethylformamide, then stirred for 18 hours while allowing the temperature to rise to 20°-25° C. The mixture is heated at 90° C. for 1 hour, cooled and poured into 3 liters of ice water containing 10 ml of concentrated hydrochloric acid (final pH about 5). The resulting precipitate is collected by filtration and the filtrate is extracted several times with 600 ml portions of dichloromethane. The precipitate is dissolved in 1 liter of dichloromethane and the solution is combined with the above dichloromethane extracts. The combined dichloromethane solution is washed with water, dried and evaporated. The residual oil is triturated with methanol and the resulting solid is collected, dissolved in tetrahydrofuran and the solution is decolorized by passage through an activated magnesium silicate column (Florisil R). The filtrate is concentrated to a small volume, diluted with ethanol and chilled. The resulting crystalline precipitate of 4,9-dihydro-4-methyl-9-oxo-pyrazolo[5,1-b]quinazoline-2-carboxylic acid, ethyl ester is collected by filtration; mp 239°-241° C., after recrystallization from dichloromethane/ethanol. to rise to +10° C is recooled to -10° C. treated dropwise to rise to 20°-25° C cooled The resulting precipitate is collected by filtration the filtrate is extracted several times with 600 ml portions of dichloromethane The precipitate is dissolved in 1 liter of dichloromethane The combined dichloromethane solution is washed with water dried evaporated The residual oil is triturated with methanol the resulting solid is collected dissolved in tetrahydrofuran The filtrate is concentrated to a small volume diluted with ethanol chilled The resulting crystalline precipitate of 4,9-dihydro-4-methyl-9-oxo-pyrazolo[5,1-b]quinazoline-2-carboxylic acid, ethyl ester is collected by filtration mp 239°-241° C., after recrystallization from dichloromethane/ethanol

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: Tert-butyldimethylsilyl chloride (6.05 g, 40.15 mmol) and imidazole (2.92 g, 42.82 mmol) were added to a stirred solution of ethyl 5-oxo-4,5-dihydro-1H-pyrazole-3-carboxylate (4.18 g, 26.76 mmol) in ACN (90 mL). The mixture was stirred at room temperature for 30 minutes, then diluted with water and extracted with AcOEt. The organic layer was separated, dried (Na2SO4), filtered and the solvents evaporated in vacuo to yield ethyl 5-{[tert-butyl(dimethyl)silyl]oxy}-1H-pyrazole-3-carboxylate (3.2 g, 44% yield) as a yellow solid, that was used in the next step without further purification. extracted with AcOEt The organic layer was separated dried (Na2SO4) filtered the solvents evaporated in vacuo

反応条件: temp:0.00; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: Tert-butyldimethylsilyl chloride (6.05 g, 40.15 mmol) and imidazole (2.92 g, 42.82 mmol) were added to a stirred solution of ethyl 5-oxo-4,5-dihydro-1H-pyrazole-3-carboxylate (4.18 g, 26.76 mmol) in ACN (90 mL). The mixture was stirred at room temperature for 30 minutes, then diluted with water and extracted with AcOEt. The organic layer was separated, dried (Na2SO4), filtered and the solvents evaporated in vacuo to yield ethyl 5-{[tert-butyl(dimethyl)silyl]oxy}-1H-pyrazole-3-carboxylate (3.2 g, 44% yield) as a yellow solid, that was used in the next step without further purification. extracted with AcOEt The organic layer was separated dried (Na2SO4) filtered the solvents evaporated in vacuo

反応条件: temp:90.01; pressure:0.00; stir:; See reaction.notes.procedure_details.

実験手順: A stirred suspension of 5.3 g of a 50% mineral oil dispersion of sodium hydride in 30 ml of dimethylformamide is treated dropwise at -10° C., under a nitrogen atmosphere, with a solution of 15.6 g of 5-oxo-2-pyrazoline-3-carboxylic acid, ethyl ester, in 65 ml of dimethylformamide. After the evolution of hydrogen ceases, the mixture is stirred for 2 hours while allowing the temperature to rise to +10° C. The mixture is recooled to -10° C. and treated dropwise, with stirring, with a solution of 21.6 g of 90% N-methylisatoic anhydride in 150 ml of dimethylformamide, then stirred for 18 hours while allowing the temperature to rise to 20°-25° C. The mixture is heated at 90° C. for 1 hour, cooled and poured into 3 liters of ice water containing 10 ml of concentrated hydrochloric acid (final pH about 5). The resulting precipitate is collected by filtration and the filtrate is extracted several times with 600 ml portions of dichloromethane. The precipitate is dissolved in 1 liter of dichloromethane and the solution is combined with the above dichloromethane extracts. The combined dichloromethane solution is washed with water, dried and evaporated. The residual oil is triturated with methanol and the resulting solid is collected, dissolved in tetrahydrofuran and the solution is decolorized by passage through an activated magnesium silicate column (Florisil R). The filtrate is concentrated to a small volume, diluted with ethanol and chilled. The resulting crystalline precipitate of 4,9-dihydro-4-methyl-9-oxo-pyrazolo[5,1-b]quinazoline-2-carboxylic acid, ethyl ester is collected by filtration; mp 239°-241° C., after recrystallization from dichloromethane/ethanol. to rise to +10° C is recooled to -10° C. treated dropwise to rise to 20°-25° C cooled The resulting precipitate is collected by filtration the filtrate is extracted several times with 600 ml portions of dichloromethane The precipitate is dissolved in 1 liter of dichloromethane The combined dichloromethane solution is washed with water dried evaporated The residual oil is triturated with methanol the resulting solid is collected dissolved in tetrahydrofuran The filtrate is concentrated to a small volume diluted with ethanol chilled The resulting crystalline precipitate of 4,9-dihydro-4-methyl-9-oxo-pyrazolo[5,1-b]quinazoline-2-carboxylic acid, ethyl ester is collected by filtration mp 239°-241° C., after recrystallization from dichloromethane/ethanol